Application of SPR imaging biosensor measurement of 20S proteasomes in blood plasma of children with thermal injury
PBN-AR
Instytucja
Wydział Biologiczno-Chemiczny (Uniwersytet w Białymstoku)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
Annals of Clinical & Laboratory Science
ISSN
0091-7370
EISSN
1550-8080
Wydawca
Association of Clinical Scientists
DOI
Rok publikacji
2016
Numer zeszytu
4
Strony od-do
407-411
Numer tomu
46
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0,8
Słowa kluczowe
angielski
Surface Plasmon Resonance Imaging
burns
children
circulating 20S proteasome
plasma
proteasomes
Streszczenia
Język
angielski
Treść
BACKGROUND: Determination of proteasome concentration in blood plasma in children with burns, with the Surface Plasmon Resonance Imaging biosensor. MATERIAL AND METHODS: 35 children scalded by hot water, with burns in 4-20% TBSA were included into the study (age 9 months up to 14 years, mean age 2,5+1 years). Blood plasma 20S proteasome concentration was assessed in 2-6h, 12-16h, 3d, 5d, and 7d after injury using Surface Plasmon Resonance Imaging biosensor. RESULTS: Statistically significant elevation of circulating 20S proteasome concentration was noted in all groups 12-16 hours after the injury; however, differences were more evident in children with the more severe burns. Circulating 20S proteasome concentration in the plasma was increased until day 7 in the group with severe burns, until day 5 in the group with moderate burns and until day 3 in the group with minor burns. Circulating 20S proteasome concentration in the plasma was highly correlated with burn severity. CONCLUSIONS: The SPR imaging biosensor can be used for determination of 20S proteasome in plasma of patients with burns. Circulating 20S proteasomes are elevated after burn injury and slowly reach the normal range during the wound healing process. Increase in circulating 20S proteasome concentration is proportional to the severity of the burn injury and do not correlate with age or sex.
Cechy publikacji
publikacja naukowa oryginalna
Inne
System-identifier
PX-58b01befd5dec03e45d28c2c