A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair
PBN-AR
Instytucja
Wydział Inżynierii Materiałowej (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Biomaterials Applications
ISSN
0885-3282
EISSN
Wydawca
Sage Publications Ltd.
DOI
Rok publikacji
2016
Numer zeszytu
6
Strony od-do
873-885
Numer tomu
30
Identyfikator DOI
Liczba arkuszy
0.6
Autorzy
Pozostali autorzy
+ 7
Słowa kluczowe
en
Scaffold; cartilage; chondrogenesis; rapid prototyping; nanofibres; chitosan
Streszczenia
Język
en
Treść
The aim was to develop a hybrid three-dimensional-tissue engineering construct for chondrogenesis. The hypothesis was that they support chondrogenesis. A biodegradable, highly porous polycaprolactone-grate was produced by solid freeform fabrication. The polycaprolactone support was coated with a chitosan/polyethylene oxide nanofibre sheet produced by electrospinning. Transforming growth factor-3-induced chondrogenesis was followed using the following markers: sex determining region Y/-box 9, runt-related transcription factor 2 and collagen II and X in quantitative real-time polymerase chain reaction, histology and immunostaining. A polycaprolactone-grate and an optimized chitosan/polyethylene oxide nanofibre sheet supported cellular aggregation, chondrogenesis and matrix formation. In tissue engineering constructs, the sheets were seeded first with mesenchymal stem cells and then piled up according to the lasagne principle. The advantages of such a construct are (1) the cells do not need to migrate to the tissue engineering construct and therefore pore size and interconnectivity problems are omitted and (2) the cell-tight nanofibre sheet and collagen-fibre network mimic a cell culture platform for mesenchymal stem cells/chondrocytes (preventing escape) and hinders in-growth of fibroblasts and fibrous scarring (preventing capture). This allows time for the slowly progressing, multiphase true cartilage regeneration.
Inne
System-identifier
WUT979d2b62d2c742b39faf558829540818
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