Pentatricopeptide motifs in the N-terminal extension domain of yeast mitochondrial RNA polymerase Rpo41p are not essential for its function
PBN-AR
Instytucja
Wydział Biologii (Uniwersytet Warszawski)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
BIOCHEMISTRY-MOSCOW
ISSN
0006-2979
EISSN
0320-9725
Wydawca
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI
URL
Rok publikacji
2016
Numer zeszytu
10
Strony od-do
1101-1110
Numer tomu
81
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 2)
Słowa kluczowe
en
mitochondria
RNA polymerase
pentatricopeptide motifs
yeast
Streszczenia
Język
en
Treść
The core mitochondrial RNA polymerase is a single-subunit enzyme that in yeast Saccharomyces cerevisiae is encoded by the nuclear RPO41 gene. It is an evolutionary descendant of the bacteriophage RNA polymerases, but it includes an additional unconserved N-terminal extension (NTE) domain that is unique to the organellar enzymes. This domain mediates interactions between the polymerase and accessory regulatory factors, such as yeast Sls1p and Nam1p. Previous studies demonstrated that deletion of the entire NTE domain results only in a temperature-dependent respiratory deficiency. Several sequences related to the pentatricopeptide (PPR) motifs were identified in silico in Rpo41p, three of which are located in the NTE domain. PPR repeat proteins are a large family of organellar RNA-binding factors, mostly involved in posttranscriptional gene expression mechanisms. To study their function, we analyzed the phenotype of strains bearing Rpo41p variants where each of these motifs was deleted. We found that deletion of any of the three PPR motifs in the NTE domain does not affect respiratory growth at normal temperature, and it results in a moderate decrease in mtDNA stability. Steady-state levels of COX1 and COX2 mRNAs are also moderately affected. Only the deletion of the second motif results in a partial respiratory deficiency, manifested only at elevated temperature. Our results thus indicate that the PPR motifs do not play an essential role in the function of the NTE domain of the mitochondrial RNA polymerase.
Cechy publikacji
discipline:Biochemia – dziedzina nauk biologicznych
discipline:Biologia
discipline:Biochemistry – field of biological sciences
discipline:Biology
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:754063
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