Variability in the oligosaccharide concentration in seeds of the map ping population of pea (Pisum sativum L.)
PBN-AR
Instytucja
Wydział Biologii i Biotechnologii (Uniwersytet Warmińsko-Mazurski w Olsztynie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Czech Journal of Genetics and Plant Breeding
ISSN
1212-1975
EISSN
Wydawca
CZECH ACAD AGRIC SCI
DOI
URL
Rok publikacji
2014
Numer zeszytu
2
Strony od-do
157-162
Numer tomu
50
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 7)
Pozostali autorzy
+ 6
Autorzy przekładu
(liczba autorów przekładu: 0)
Słowa kluczowe
en
genetic map; molecular markers; QTL; rugosus (r) locus; seed oligosaccharide
Streszczenia
Język
en
Treść
Anti-nutritional compounds are among the obstacles to the use of pea seeds as a protein source in both feed and food. These compounds are poorly digested by both monogastric animals and humans. There are three main oligosaccharides in pea: raffinose, stachyose and verbascose (raffinose family oligosaccharides – RFOs). The concentration of oligosaccharides in dry seeds, the oligosaccharide percent to the total content of soluble sugars and quantitative trait loci (QTLs) were analysed in the mapping population Wt10245 × Wt11238. The composition and concentration of soluble carbohydrates in seeds harvested from two field experiments (2002 and 2004) were analysed by the high resolution gas chromatography method. The Wt10245 × Wt11238 population was chosen because of the greater difference in the concentration of RFOs in seeds between parental lines (56.48 mg/g seed in Wt10245 and 99.1 mg/g seed in Wt11238). The average levels of oligosaccharides (mg/g seed) from both field experiments in the mapping population were: myo-inositol 1.5, sucrose 33.3, galactinol 0.8, raffinose 9.6, stachyose 30.1, verbascose 37.1. The total oligosaccharide concentration was 76.8 mg/g seed. This comprised anaverage of 68% soluble sugars, with the range from 59% to 75%. There was no interaction between lines and years of experiments (significance of lines × year interaction, F statistic > 0.01). One main quantitative trait locus was found for both experiments in LG VA (the tl-r interval) and three additional: in LG I (five traits 2002 and 2004 near afp1k), LG II (two traits 2002 near afp15h) and LG IIIB (five traits 2004 and 2002 near afp4i and M16). The main QTL was responsible for the level of RFOs and the total soluble sugar concentration in seeds. The results are in agreement with the knowledge of RFO biosynthesis. This makes selection for changes in the proportion of the particular oligosaccharides difficult, like in Phaseolus. However, it is possible to decrease the RFO content in pea seeds. The linkage between QTL and the gene r is interesting. The rugosus (r) locus changes the morphology and distribution of starch grains, decreases the total starch accumulation, produces a higher ratio of amylose to amylopectin and higher sugar and water content during development along with changes in cell size and lipid content.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
520208