Application of Celluclast 1.5L in apple pectin extraction
PBN-AR
Instytucja
Wydział Technologii Żywności (Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Carbohydrate Polymers
ISSN
0144-8617
EISSN
Wydawca
DOI
URL
Rok publikacji
2015
Numer zeszytu
Strony od-do
251-257
Numer tomu
134
Identyfikator DOI
Liczba arkuszy
0.5
Słowa kluczowe
en
Pectin, enzyme, celluclast, extraction, composition, bioactivity
Streszczenia
Język
en
Treść
Pectins were extracted from apple pomace with Celluclast 1.5L at a dose of 25, 50 and 75 μl per 1 g of material. In obtained pectin, the galacturonic acid (GalA) content, the neutral sugars (NS) profile, the degree of methylation (DM) and acetylation (DAc), the molecular mass, protein, ash and polyphenol levels as well as antioxidant and antitumor activity were determined. The lowest dose of enzymatic preparation resulted in the yield of pectin isolation comparable with acidic treatment (15.3%). Application of higher dose caused further, almost 4% increase in polymer recovery. Enzymatically isolated pectin was characterised by larger molecular mass and contained more GalA of higher DM and DAc than polymer extracted with acid. It was also richer in protein and polyphenols, and had different NS profile, which resulted in higher antiradical activity as well as the ability to inhibit the proliferation and invasion of Caco-2 adenocarcinoma cells.
Inne
System-identifier
URd5987fd63af24b65a67165ca172e131a
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