A new polymorphisms in regulatory regions of porcine u-calpain gene and their association with CAPN1 transcript abundance.
PBN-AR
Instytucja
Instytut Zootechniki - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Annals of Animal Science
ISSN
1642-3402
EISSN
2300-8733
Wydawca
IZ PIB Kraków
DOI
Rok publikacji
2014
Numer zeszytu
3
Strony od-do
525-535
Numer tomu
14
Identyfikator DOI
Liczba arkuszy
1.00
Słowa kluczowe
EN
calpain
CAPN1 gene
polymorphism
expression level
pig
Open access
Tryb otwartego dostępu
Inne
Wersja tekstu w otwartym dostępie
Wersja opublikowana
Licencja otwartego dostępu
Inna
Czas opublikowania w otwartym dostępie
Razem z publikacją
Streszczenia
Język
EN
Treść
The activity of calpains, in particular mu-calpain, is associated with several processes occurring in muscle tissue postmortem and influences meat quality parameters. Therefore, the CAPN1 gene coding for large subunit of mu-calpain is considered as a candidate gene associated with meat quality traits. The aim of our study was to identify new polymorphisms in regulatory regions of the porcine CAPN1 gene and to estimate their impact on CAPN1 transcript abundance. In the present study, 7 polymorphisms in the porcine CAPN1 gene were identified, of which 5 were localized in introns (g.1195_1197insCCT; g.1429G>A; g.[4479A>G; 4526A>T; 4529_4530delAG]), one in 3'untranslated region (g.25676C>T) and one microsatellite sequence in promoter region (c.-155AGGG[ 3_5]). The analysed populations (a total of 451 gilts representing three pure breeds: Pietrain, Polish Landrace, Polish Large White and one conservation breed Pulawska) were not in Hardy-Weinberg equilibrium according to mutation g.25676C>T in 3'untranslated region (all breeds), g.1429G>A (Pulawska pigs) and g.[4479A>G; 4526A>T; 4529_4530delAG] (PLW pigs). Furthermore, the analysed SNPs in the porcine CAPN1 gene were in linkage disequilibrium (P <= 0.05). The CAPN1 transcript abundance was also estimated in two important muscles (m. longissimus dorsi, m. semimembranosus). In longissimus dorsi muscle, a significant effect of c.-155AGGG[3_5] polymorphism in promoter region on CAPN1 expression levels was determined. The c.-155AGGG[3/3] pigs showed a statistically higher (P <= 0.05) expression level of the CAPN1 gene when compared to c.-155AGGG[4/4] homozygotes. The results obtained suggested that detected SNPs within regulatory regions of the CAPN1 gene could be related to transcript level and activity of mu-calpain. The selected polymorphisms are proposed to be associated with meat production traits.
Cechy publikacji
Oryginalny-artykuł-naukowy
Inne
System-identifier
4500
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych