Platelet-Specific Chemokines Contribute to the Pathogenesis of Acute Lung Injury
PBN-AR
Instytucja
Instytut Biologii Medycznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY (35pkt w roku publikacji)
ISSN
1044-1549
EISSN
1535-4989
Wydawca
AMER THORACIC SOC
DOI
URL
Rok publikacji
2017
Numer zeszytu
2
Strony od-do
261-270
Numer tomu
56
Link do pełnego tekstu
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Autorzy
Pozostali autorzy
+ 8
Słowa kluczowe
en
acute lung injury; platelets; chemokines
Streszczenia
Język
en
Treść
Platelets and neutrophils contribute to the development of acute lung injury (ALI). However, the mechanism by which platelets make this contribution is incompletely understood. We investigated whether the two most abundant platelet chemokines, CXCL7, which induces neutrophil chemotaxis and activation, and CXCL4, which does neither, mediate ALI through complementary pathogenic pathways. To examine the role of platelet-derived chemokines in the pathogenesis of ALI using Cxcl7(-/-) and Cxcl4(-/-) knockout mice and mice that express human CXCL7 or CXCL4, we measured levels of chemokines in these mice. ALI was then induced by acid aspiration, and the severity of injury was evaluated by histology and by the presence of neutrophils and protein in the bronchoalveolar lavage fluid. Pulmonary vascular permeability was studied in vivo by measuring extravasation of fluorescently labeled dextran. Murine CXCL7, both recombinant and native protein released from platelets, can be N-terminally processed by cathepsin G to yield a biologically active CXCL7 fragment. Although Cxcl7(-/-) mice are protected from lung injury through the preservation of endothelial/epithelial barrier function combined with impaired neutrophils transmigration, Cxcl4(-/-) mice are protected through improved barrier function without affecting neutrophils transmigration to the airways. Sensitivity to ALI is restored by transgenic expression of CXCL7 or CXCL4. Platelet-derived CXCL7 and CXCL4 contribute to the pathogenesis of ALI through complementary effects on neutrophil chemotaxis and through activation and vascular permeability.
Cechy publikacji
discipline:Biochemia – dziedzina nauk biologicznych
discipline:Biologia medyczna
discipline:Biochemistry – field of biological sciences
discipline:Medical biology
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:820889
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