Inhibition of blood platelet adhesion by phenolics' rich fraction of Hippophae rhamnoides L. fruits.
PBN-AR
Instytucja
Instytut Uprawy Nawożenia i Gleboznawstwa - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Physiology and Pharmacology (25pkt w roku publikacji)
ISSN
0867-5910
EISSN
1899-1505
Wydawca
POLISH PHYSIOLOGICAL SOCIETY
DOI
Rok publikacji
2017
Numer zeszytu
2
Strony od-do
223-229
Numer tomu
68
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
blood platelets
antiplatelet activity
adhesion
aggr egation
Hippophaer hamnoides
glutathione
reactive oxygen species
Streszczenia
Język
en
Treść
Beneficial influence of fruits on human health may be their ability to prevent the hyperactivation of blood platelets and cardiovascular disorders. Effects of the phenolic fraction from Hippophae rhamnoides fruit on different stages of blood platelet activation (platelet adhesion and aggregation) were studied in vitro. We also examined effects of the H. rhamnoides fraction on metabolism of thiol groups, which plays an important role in platelet functions. The effects of the H. rhamnoides fraction on adhesion of blood platelets to collagen and fibrinogen were determined with Tuszynski's and Murphy's method. The platelet aggregation was determined with turbidimetry. The action of the H. rhamnoides fraction on the level of thiol groups in platelet proteins and a level of glutathione (GSH) in platelets was estimated with 5,5'-dithio-bis(2-nitro-benzoic acid). The tested fraction of H. rhamnoides (0.5 - 50 μg/ml; 30 min of the incubation time 30 min) inhibited blood platelets adhesion to collagen and fibrinogen. The effect of the tested fraction on blood platelet adhesion depended on concentration of fraction. In presence of the highest tested concentration which was 50 μg/ml, inhibition of platelet adhesion for thrombin-activated platelets was about 55%. On the other hand, tested plant fraction had no anti-aggregatory properties. Our results showed anti-adhesive properties of phenolic fraction from H. rhamnoides fruit and we suggest that it may be beneficial for prevention of cardiovascular diseases.
Cechy publikacji
Medycyna
Nauki farmaceutyczne
Medicine
Pharmacy
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:822898