Novel properties of Hippophae rhamnoides L. twig and leaf extracts - anti-virulence action and synergy with antifungals studied in vitro on Candida spp. model
PBN-AR
Instytucja
Instytut Uprawy Nawożenia i Gleboznawstwa - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
MICROBIAL PATHOGENESIS (20pkt w roku publikacji)
ISSN
0882-4010
EISSN
Wydawca
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI
URL
Rok publikacji
2017
Numer zeszytu
Strony od-do
372-379
Numer tomu
107
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Hippophae rhamnoides
Twig and leaf extracts
Synergy with antimicrobials
Morphogenesis
Anti-biofilm
Streszczenia
Język
en
Treść
Original, chemically characterized Sea buckthorn (SBT) twig and leaf extracts were in vitro studied in terms of anti-Candida activity. Minimum inhibitory concentrations (MICs) of the extracts against C. albicans ATCC 10231 ranged: 250 μg/ml (twig), 31.5 μg/ml (leaf), and against C. glabrata G1 (clinical isolate) - 15.6 μg/ml (twig), 3.9 μg/ml (leaf). Next the extracts have been used at their subMIC. Both extracts significantly enhanced activity of fluconazole (FLC) and caspofungin (CAS) against C. albicans and increased their efficacy against C. glabrata, measured by an agar dilution assay combined with the E-test. The extracts inhibited C. albicans morphogenesis such as germ tube and hyphae formation as well as invasion to the “Spider” Agar. Antiadhesive and anti-biofilm activities of the extracts were evaluated by Alamar Blue reduction assay. It showed not significant reduction in the degree of cell adhesion (by 10–15%) but noticeable decrease of biofilm formation (by 80% in the case of SBT-twig extract). In conclusion, this study provided the evidence that SBT extracts, used at non-cytotoxic concentrations for the fibroblasts (IC50 from 664.8 μg/ml to 1060.4 μg/ml), targeted some of Candida spp. virulence factors essential for the establishment of the infection. SBT twigs, previously regarded as waste material, were shown to be also a valuable source of the substances with promising antimicrobial activity.
Cechy publikacji
Original article
Oryginalny artykuł naukowy
Inne
System-identifier
PBN-R:822902
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