Presence of Pathogenic Rickettsiae and Protozoan in Samples of Raw Milk from Cows, Goats, and Sheep
PBN-AR
Instytucja
Instytut Medycyny Wsi im. Witolda Chodźki
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Foodborne Pathogens and Disease (30pkt w roku publikacji)
ISSN
1535-3141
EISSN
1556-7125
Wydawca
MARY ANN LIEBERT INC
DOI
URL
Rok publikacji
2017
Numer zeszytu
4
Strony od-do
189-194
Numer tomu
14
Identyfikator DOI
Liczba arkuszy
1.00
Słowa kluczowe
en
raw milk
Toxoplasma gondii
Coxiella burnetii
Anaplasma phagocytophilum
Rickettsia spp.
Psychrobacter alimentarius
PCR Introduction
Streszczenia
Język
en
Treść
Objective: The aim of the present work was to determine the presence of various rickettsiae and protozoan in raw milk and the assessment the potential, milk-borne route in the spread of selected zoonotic pathogens. Materials: A total of 119 raw milk samples collected randomly from 63 cows, 29 goats, and 27 sheep bred on 34 farms situated on eight communities in eastern Poland were examined by polymerase chain reaction (PCR) method for the presence of pathogenic rickettsiae (Coxiella burnetii, Anaplasma phagocytophilum, and Rickettsia spp.) and protozoan (Toxoplasma gondii). Results: The only prevalent pathogen was T. gondii, which was found in 10 samples of cow milk (15.9%), in one sample of goat milk (3.4%), and in one sample of sheep milk (3.7%). One sample of cow milk was positive for C. burnetii; however, the sequence analysis did not confirm any species of Coxiella or Coxiella-like organisms, but showed 100% homology to Psychrobacter alimentarius. None of the examined samples showed the presence of A. phagocytophilum or Rickettsia spp. Conclusions: The results of this study suggest a potential hazard of milk-borne Toxoplasma infection, mostly by consumption of raw cow milk. The milk-borne spread seems to be limited or nonsignificant in the case of C. burnetii, A. phagocytophilum, and Rickettsia spp. The false-positive sample for Coxiella spp. suggests that some care should be taken in the interpretation of the results obtained by using the PCR method.
Cechy publikacji
discipline:Biotechnologia – dziedzina nauk biologicznych
discipline:Nauki o zdrowiu
discipline:Biotechnology – field of biological sciences
discipline:Health sciences
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:813771
CrossrefMetadata from Crossref logo
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