Antioxidant activity and phenolic composition of a red bean (Phasoelus vulgaris) extract and its fractions
PBN-AR
Instytucja
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Natural Product Communications (20pkt w roku publikacji)
ISSN
1934-578X
EISSN
1555-9475
Wydawca
DOI
URL
Rok publikacji
2017
Numer zeszytu
4
Strony od-do
541-544
Numer tomu
12
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Red bean
extract
phenolic compounds
tannins
antioxidant activity
HPLC-MS
Streszczenia
Język
en
Treść
The activities of the crude acetonic extract of red bean and its two fractions were determined using a β-carotene-linoleate model system as well as the total antioxidant activity (TAA), the total phenolics content (TPC), the DPPH radical-scavenging activity, and the reducing power assays. Results from the in vitro assays showed the highest values when tannins (fraction II) were tested. Specifically, the TAA of the tannins fraction was 4.37 mmol Trolox eq./g fraction; whereas, the crude extract and fraction I were 0.481 and 0.093 μmol Trolox eq./mg extract or fraction, respectively. The content of total phenolics in fraction II was the utmost (612 mg/g); the tannins content, assayed by the vanillin method and expressed as absorbance units at 500 nm per 1 g, was 938. RP-HPLCPAD- MS profiling revealed the presence of 33 compounds: quercetin arabinoglucoside, quercetin rutinoside, quercetin, p-hydroxybenzoic acid, and kaempferol rutinoside were the most abundant phenolics in the extract.
Inne
System-identifier
252-001275