Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation
PBN-AR
Instytucja
Wydział Technologii Żywności (Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Genes and Nutrition
ISSN
1555-8932
EISSN
Wydawca
DOI
URL
Rok publikacji
2016
Numer zeszytu
11:22
Strony od-do
1-12
Numer tomu
Identyfikator DOI
Liczba arkuszy
0.55
Autorzy
Pozostali autorzy
+ 3
Słowa kluczowe
en
AKT/mTOR pathway, CLA-enriched egg yolks, Cancer chemoprevention, DNA microarray, MCF-7 cancer cells, SFA/MUFA, Transcriptome
Streszczenia
Język
en
Treść
BACKGROUND: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology. RESULTS: We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes. CONCLUSIONS: We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens' diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent.
Inne
System-identifier
URd0fbe5b3921c47e9872f30bab1499139
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