Determination of collagen type IV by surface plasmon resonance imaging using a specific biosensor
PBN-AR
Instytucja
Wydział Biologiczno-Chemiczny (Uniwersytet w Białymstoku)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
Analytical Biochemistry
ISSN
0003-2697
EISSN
0003-2697
Wydawca
Elsevier
DOI
URL
Rok publikacji
2016
Numer zeszytu
Strony od-do
40-46
Numer tomu
515
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0,9
Autorzy
(liczba autorów: 4)
Pozostali autorzy
+ 2
Słowa kluczowe
angielski
Biosensor
Collagen IV
Surface Plasmon Resonance Imaging
Streszczenia
Język
angielski
Treść
Serum collagen type IV (COLIV) is a promising tumor marker. High COLIV concentrations have been found in the serum of patients with colorectal, gastric, lung, liver and breast cancers. The aim of this work was to develop a biosensor for use with the Surface Plasmon Resonance Imaging (SPRI) technique for COLIV determination. The biosensor consists of glass covered with gold and immobilized monoclonal mouse anti-human collagen type IV antibody via cysteamine linker. The biosensor works selectively within a dynamic response range between 10 and 300 ng mL-1, with LOD 2.4 ng mL-1 and LOQ 8 ng mL-1. The precision of determination is 4.7% at a 150 ng mL-1 COLIV spike and 8.0% at a 20 ng mL-1 spike, with recoveries of 101% and 106% respectively. A 100-fold excess of collagen I, albumin, laminin and fibronectin is tolerated. The average COLIV blood plasma concentration of healthy donors determined by the developed method was 69 ± 10 ng mL-1, while the median of six results available in the literature was approximately 80 ng mL-1. The average COLIV blood plasma concentration of breast cancer patients was 360 ± 68 ng mL-1, showing the high potential of COLIV as a marker of this type of cancer.
Cechy publikacji
publikacja naukowa oryginalna
Inne
System-identifier
000040417
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