Altered expression of the PLAGL1 (ZAC1/LOT1) gene in colorectal cancer: Correlations to the clinicopathological parameters
PBN-AR
Instytucja
Wydział Lekarski (Uniwersytet Warmińsko-Mazurski w Olsztynie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
International Journal of Oncology
ISSN
1019-6439
EISSN
1791-2423
Wydawca
SPANDIDOS PUBL LTD
DOI
URL
Rok publikacji
2015
Numer zeszytu
3
Strony od-do
951-962
Numer tomu
47
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
PLAGL1 expression
colorectal cancer
healthy colon mucosa
clinicopathological parameters
survival
qPCR
western blotting
IHC
Streszczenia
Język
en
Treść
Pleomorphic adenoma gene-like 1 gene (PLAGL1) encodes a zinc-finger nuclear transcription factor which promotes apoptosis and cell cycle arrest. Loss or downregulation of its expression has been observed in various human neoplasms. This study compared PLAGL1 expression in colorectal cancer (CRC) tissue and colon mucosa of healthy subjects at the mRNA and protein levels, and estimated its prognostic value. The PLAGL1 mRNA levels were also determined in CRC cell lines. We collected paired tumor tissue and unchanged mucosa of the large intestine from 121 CRC patients as well as 72 colon biopsies of healthy subjects obtained during screening colonoscopy. PLAGL1 mRNA levels were determined by quantitative PCR, while PLAGL1 protein expression was estimated by western blotting and immunohistochemistry. PLAGL1 mRNA level in tumor tissue was ~2-fold lower than in samples of corresponding unchanged tissues and biopsies of healthy colon mucosa. Downregulated expression of PLAGL1 mRNA was also observed in all tested CRC cell lines. Although the average content of PLAGL1 protein did not differ significantly between tumor and unchanged tissues of CRC patients or colon mucosa of healthy individuals, the decreased PLAGL1 protein levels in tumor specimens correlated with lymph node involvement, the presence of metastases and higher TNM disease stage. The PLAGL1 expression level did not correlate significantly with patient overall survival; however, the hazard ratio for patients whose tumor tissues showed reduced PLAGL1 immunohistochemical staining was twice higher than in patients with increased PLAGL1 immunoreactivity. In conclusion, these results suggest that dysregulation of PLAGL1 expression may be involved to some extent in the progression of CRC, but the so far collected patient survival data do not confirm applicability of the PLAGL1 expression level as a prognostic factor in CRC.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
EX-56b4a0cf8106eb71826fe8b5
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