Nano-characterization of two closely related melanoma cell lines with different metastatic potential
PBN-AR
Instytucja
Instytut Fizyki Jądrowej im. Henryka Niewodniczańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
ISSN
0175-7571
EISSN
Wydawca
DOI
URL
Rok publikacji
2015
Numer zeszytu
1-2
Strony od-do
49-55
Numer tomu
44
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 5
Słowa kluczowe
en
Melanoma cells
Cell elasticity
Combined AFM/fluorescence microscopy
Correlation between surface morphology and cellular stiffness
ATOMIC-FORCE MICROSCOPY
CYTOSKELETON
ADHESION
CANCER
PROLIFERATION
STIFFNESS
VARIANTS
Streszczenia
Język
en
Treść
Cutaneous malignant melanoma is one of the most lethal types of skin cancer. Its progression passes through several steps, leading to the appearance of a new population of cells with aggressive biological potential. Here, we focused on the nano-characterization of two different melanoma cell lines with similar morphological appearance but different metastatic potential, namely, WM115 from vertical growth phase (VGP) and WM266-4 derived from metastasis to skin. The first cell line represents cells that progressed to the VGP, while the WM266-4 cell line denotes cells from the metastasis to skin. Exploring with a combination of atomic force and fluorescence microscopes, our goal was to identify cell surface characteristics in both cell lines that may determine differences in the cellular nano-mechanical properties. Cell elasticity was found to be affected by the presence of F-actin-based flexible ridges, rich in F-actin co-localized with beta 1 integrins in the studied cell lines. These results point out how progressive changes in the surface structure of melanoma cells can affect their bionanomechanical properties.
Cechy publikacji
peer-reviewed
original-article
Inne
System-identifier
24722
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych