Polyprenols are Synthesized by a Plastidial cis-Prenyltransferase and Influence Photosynthetic Performance
PBN-AR
Instytucja
Instytut Chemii Organicznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
PLANT CELL (45pkt w roku publikacji)
ISSN
1040-4651
EISSN
Wydawca
AMER SOC PLANT BIOLOGISTS
DOI
URL
Rok publikacji
2017
Numer zeszytu
Strony od-do
Numer tomu
Identyfikator DOI
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Streszczenia
Język
en
Treść
Plants accumulate a family of hydrophobic polymers known as polyprenols, yet how they are synthesized, where they reside in the cell, and what role they serve is largely unknown. Using Arabidopsis thaliana as a model, we present evidence for the involvement of a plastidial cis-prenyltransferase (AtCPT7) in polyprenol synthesis. Gene inactivation and RNAi-mediated knockdown of AtCPT7 eliminated leaf polyprenols while its overexpression increased their content. Complementation tests in the polyprenol-deficient yeast ∆rer2 mutant and enzyme assays with recombinant AtCPT7 confirmed that the enzyme synthesizes polyprenols of approximately 55 carbons in length using geranylgeranyl diphosphate (GGPP) and isopentenyl diphosphate (IPP) as substrates. Immunodetection and in vivo localization of AtCPT7 fluorescent protein fusions showed that AtCPT7 resides in the stroma of mesophyll chloroplasts. The enzymatic products of AtCPT7 accumulate in thylakoid membranes and in their absence, thylakoids adopt an increasingly \textquoterightfluid membrane\textquoteright state. Chlorophyll fluorescence measurements from the leaves of polyprenol-deficient plants revealed impaired photosystem II operating efficiency, and their thylakoids exhibited a decreased rate of electron transport. These results establish that (i) plastidial AtCPT7 extends the length of GGPP to ~55 carbons, which then accumulate in thylakoid membranes; and (ii) these polyprenols influence photosynthetic performance through their modulation of thylakoid membrane dynamics.
Cechy publikacji
Original article
Original article presents the results of original research or experiment.
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Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
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System-identifier
PBN-R:857890
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