Agreement between commercial assays for haptoglobin and serum amyloid A in goats.
PBN-AR
Instytucja
Wydział Medycyny Weterynaryjnej (Szkoła Główna Gospodarstwa Wiejskiego w Warszawie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
ACTA VETERINARIA SCANDINAVICA
ISSN
1751-0147
EISSN
Wydawca
BIOMED CENTRAL LTD
DOI
URL
Rok publikacji
2017
Numer zeszytu
1
Strony od-do
65
Numer tomu
59
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
angielski
Acute phase proteins
ELISA
Small ruminants
Streszczenia
Język
angielski
Treść
Haptoglobin (Hp) and serum amyloid A (SAA) are considered as the major acute phase proteins (APPs) in goats. These APPs have been investigated in several studies during the last decade. In most studies, a colorimetric assay for Hp and a solid phase sandwich ELISA for SAA have been used for quantification. In 2015, reference intervals for APPs were determined using a new type of assay, the competitive ELISA (cELISA). Results obtained by the cELISA differed significantly from results obtained by previously used assays. The present study aimed to assess the agreement between so far used assays and cELISAs. Sera of 152 female dairy goats of two Polish national breeds were analysed. The concentration of Hp was determined using a colorimetric assay (Hp-CA) and the cELISA (Hp-cELISA), while a solid phase sandwich ELISA (SAA-sELISA) and the cELISA (SAA-cELISA) were used to measure SAA. Agreement between test results was assessed by preparing Bland–Altman plots, and analyzing 95% limits of agreement (LoA). Finally, the assays for Hp and SAA were compared using 147 and 138 serum samples, respectively, as 5 and 14 paired measurements, respectively, were excluded from agreement analyses to avoid extrapolation of Hp and SAA concentration. Measurements obtained by the Hp-CA and Hp-cELISA showed weak positive correlation (r = 0.24, P = 0.003). Limits of agreement (LoA) ranged from + 1.6 (95% CI 1.4 to 1.8) g/L to − 1.5 (95% CI − 1.7 to − 1.3) g/L. Measurements yielded by the SAA-sELISA and SAA-cELISA did not correlate (r = − 0.01, P = 0.855). LoA ranged from + 14.5 mg/L (95% CI 12.9 to 16.1) to − 8.5 mg/L (95% CI − 10.1 to − 6.9). Agreement between the two types of commercial assays for determination of Hp and SAA concentrations in goats is poor and cELISAs tend to underrate both Hp and SAA concentrations
Cechy publikacji
discipline:Nauki weterynaryjne
discipline:Veterinary sciences
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:833625
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