Biosynthesis of gonadotropin releasing hormone (GnRH) and GnRH receptor (GnRHR) in hypothalamic-pituitary unit of anestrous and cyclic ewes
PBN-AR
Instytucja
Muzeum i Instytut Zoologii Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (20pkt w roku publikacji)
ISSN
0008-4212
EISSN
Wydawca
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI
URL
Rok publikacji
2017
Numer zeszytu
2
Strony od-do
178-184
Numer tomu
95
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 6)
Pozostali autorzy
+ 5
Autorzy przekładu
(liczba autorów przekładu: 0)
Słowa kluczowe
angielski
ewe
seasonality
hypothalamus
pituitary
GnRH
GnRHR
LH
Streszczenia
Język
angielski
Treść
This study was performed to explain how the molecular processes governing the biosynthesis of gonadotropinreleasing hormone (GnRH) and GnRH receptor (GnRHR) in the hypothalamic-pituitary unit are reflected by luteinizing hormone (LH) secretion in sheep during anoestrous period and during luteal and follicular phases of the oestrous cycle. Using an enzyme-linked immunosorbent assay (ELISA), we analyzed the levels of GnRH and GnRHR in preoptic area (POA), anterior (AH) and ventromedial hypothalamus (VM), stalk-median eminence (SME), and GnRHR in the anterior pituitary gland (AP). Radioimmunoassay has also been used to define changes in plasma LH concentrations. The study provides evidence that the levels of GnRH in the whole hypothalamus of anoestrous ewes were lower than that in sheep during the follicular phase of the oestrous cycle (POA: p < 0.001, AH: p < 0.001, VM: p < 0.01, SME: p < 0.001) and not always than in luteal phase animals (POA: p < 0.05, SME: p < 0.05). It has also been demonstrated that the GnRHR amount in the hypothalamus-anterior pituitary unit, as well as LH level, in the blood in anoestrous ewes were significantly lower than those detected in animals of both cyclic groups. Our data suggest that decrease in LH secretion during the long photoperiod in sheep may be due to low translational activity of genes encoding both GnRH and GnRHR.
Inne
System-identifier
PX-5a783a20d5dea664c71f839e
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych