Enhanced cytocompatibility and osteoinductive properties of sol–gel-derived silica/zirconium dioxide coatings by metformin functionalization
PBN-AR
Instytucja
Wydział Biologii i Hodowli Zwierząt (Uniwersytet Przyrodniczy we Wrocławiu)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
JOURNAL OF BIOMATERIALS APPLICATIONS (30pkt w roku publikacji)
ISSN
0885-3282
EISSN
Wydawca
SAGE PUBLICATIONS LTD
DOI
URL
Rok publikacji
2017
Numer zeszytu
5
Strony od-do
570-586
Numer tomu
32
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 2
Słowa kluczowe
angielski
Metformin
adipose-derived multipotent mesenchymal stromal cells
osteogenic differentiation
silica/zirconium dioxide
sol-gel coatings
Streszczenia
Język
angielski
Treść
The aim of this study was to evaluate the pro-osteogenic properties of sol–gel-derived silica/zirconium dioxide coatings functionalized with 1 mM of metformin. The matrices were applied on 316L stainless steel using dip-coating technique. First of all, physicochemical properties of biomaterials were evaluated. Surface morphology and topography was determined using energy-dispersive X-ray spectroscopy and atomic force microscopy. The chemical composition was evaluated using Fourier transform infrared spectroscopy. Further, wettability and surface free energy were characterized. Cytocompatibility of biomaterials was tested in vitro using model of human multipotent mesenchymal stromal cells isolated from adipose tissue. The influence of biomaterials on cells morphology and proliferation was determined. Osteogenic effect of obtained biomaterials was evaluated in terms of their influence on secretory activity of human multipotent mesenchymal stromal cells isolated from adipose tissue and matrix mineralization. Analysis was performed in relation to the control cultures i.e. maintained on pure SS316L substrate and SS316L covered with silica/zirconium dioxide. Obtained results indicate that silica/zirconium dioxide_metformin coatings ameliorated metabolic and proliferative activity of human multipotent mesenchymal stromal cells isolated from adipose tissue, as well as promoted their proper growth and adhesion. The human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on biomaterials were characterized by typical fibroblast-like morphology. The addition of metformin to the silica/zirconium dioxide coatings improved functional differentiation of human multipotent mesenchymal stromal cells isolated from adipose tissue. Osteogenic cultures on silica/zirconium dioxide_metformin were characterized by formation of well-developed osteonodules rich in calcium and phosphorous. Moreover, human multipotent mesenchymal stromal cells isolated from adipose tissue cultured on silica/zirconium dioxide_metformin synthesized increased amount of alkaline phosphatase, bone morphogenetic protein 2 and osteopontin, both on messenger RNA and protein level. Obtained biomaterials modulate cellular plasticity of human multipotent mesenchymal stromal cells isolated from adipose tissue promoting their osteogenic differentiation, thus may find application in broadly defined tissue engineering.
Inne
System-identifier
PX-5a0d74bbd5de60d919fdca09
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