Gentamicin-loaded polysaccharide membranes for prevention and treatment of post-operative wound infections in the skeletal system
PBN-AR
Instytucja
Wydział Inżynierii Materiałowej i Ceramiki (Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie)
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Pharmaceutical Research (40pkt w roku publikacji)
ISSN
0724-8741
EISSN
1573-904X
Wydawca
New York : Kluwer Academic Publishers
DOI
URL
Rok publikacji
2017
Numer zeszytu
10
Strony od-do
2075--2083
Numer tomu
34
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0.64
Słowa kluczowe
EN
membranes
gentamicin
gellan gum
alginate
local drug delivery
Streszczenia
Język
EN
Treść
To develop polysaccharide-based membranes that allow controlled and localized delivery of gentamicin for the treatment of post-operative bone infections. Membranes made of gellan gum (GUM), sodium alginate (ALG), GUM and ALG crosslinked with calcium ions (GUM + Ca and ALG + Ca, respectively) as well as reference collagen (COL) were produced by freeze-drying. Mechanical properties, drug release, antimicrobial activity and cytocompatibility of the membranes were assessed. The most appropriate handling and mechanical properties (Young's modulus, E = 92 +/- 4 MPa and breaking force, F (MAX) = 2.6 +/- 0.1 N) had GUM + Ca membrane. In contrast, COL membrane showed F (MAX) = 0.14 +/- 0.02 N, E = 1.0 +/- 0.3 MPa and was deemed to be unsuitable for antibiotic delivery. The pharmacokinetic data demonstrated a uniform and sustainable delivery of gentamicin from GUM + Ca (44.4 +/- 1.3% within 3 weeks), while for COL, ALG and ALG + Ca membranes the most of the drug was released within 24 h (55.3 +/- 1.9%, 52.5 +/- 1.5% and 37.5 +/- 1.8%, respectively). Antimicrobial activity against S. aureus and S. epidermidis was confirmed for all the membranes. GUM + Ca and COL membranes supported osteoblasts growth, whereas on ALG and ALG + Ca membranes cell growth was reduced. GUM + Ca membrane holds promise for effective treatment of bone infections thanks to favorable pharmacokinetics, bactericidal activity, cytocompatibility and good mechanical properties.
Cechy publikacji
original article
peer-reviewed
Inne
System-identifier
idp:110192
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