Identification of Single Nucleotide Polymorphisms Associated with Brown Rust Resistance, α-Amylase Activity and Pre-harvest Sprouting in Rye (Secale cereale L.)
PBN-AR
Instytucja
Wydział Rolnictwa i Bioinżynierii (Uniwersytet Przyrodniczy w Poznaniu)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
PLANT MOLECULAR BIOLOGY REPORTER (40pkt w roku publikacji)
ISSN
0735-9640
EISSN
1572-9818
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2017
Numer zeszytu
3
Strony od-do
366-378
Numer tomu
35
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Candidate gene association mapping
DArTSeq
Genome-wide association mapping
Kompetitive allele specific PCR
ScBx genes
Streszczenia
Język
en
Treść
Rye is a crop with relatively high resistance to biotic and abiotic stresses. However, the resistance to brown rust (Puccinia recondita f. sp. secalis) and pre-harvest sprouting are still not satisfactory. High α-amylase activity is also among the main disadvantages of this species. Therefore, effective tools, e.g. molecular markers, allowing precise and environmentally independent selection of favourable alleles are desirable. In the present study, two kinds of association mapping—genome-wide association mapping (GWAM) based on sequences of DArTSeq markers and candidate gene association mapping (CGAM) based on sequences of ScBx genes—were chosen for development of molecular markers fulfilling these criteria. The analysed population consisted of 149 diverse inbred lines (DILs). Altogether, 67 and 11 single nucleotide polymorphisms (SNPs) identified in, respectively, GWAM and CGAM, were significantly associated with the investigated traits: 2 SNPs with resistance to brown rust, 71 SNPs with resistance to pre-harvest sprouting and 5 SNPs with α-amylase activity in the grain. Fifteen SNPs were stable across all environments. The highest number (13) of environmentally stable SNPs was associated with pre-harvest sprouting resistance. The test employing the Kompetitive Allele Specific PCR method proved the versatility of four markers identified in both GWAM and CGAM
Cechy publikacji
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:823368
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