Impact of fluoride and a static magnetic field on the gene expression that is associated with the antioxidant defense system of human fibro-blasts
PBN-AR
Instytucja
Sieć Badawcza Łukasiewicz - Instytut Napędów i Maszyn Elektrycznych KOMEL
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
CHEMICO-BIOLOGICAL INTERACTIONS (30pkt w roku publikacji)
ISSN
0009-2797
EISSN
Wydawca
ELSEVIER IRELAND LTD
DOI
URL
Rok publikacji
2018
Numer zeszytu
287
Strony od-do
13-19
Numer tomu
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
angielski
Fluoride
Static magnetic fields
Oligonucleotide microarray
Antioxidant defense system
Fibroblasts
Streszczenia
Język
angielski
Treść
Fluoride cytotoxicity has been associated with apoptosis, oxidative stress, general changes in DNA and RNA and protein biosynthesis, whereas the results of studies on the effect of SMF on antioxidant activity of cells are contradictory. Therefore, the aim of our study was to evaluate the simultaneous exposure of human cells to fluoride SMF that are generated by permanent magnets on the expression profile of the genes that are associated with the antioxidant defense system. Control fibroblasts and fibroblasts that had been treated with fluoride were subjected to the influence of SMF with a moderate induction. In order to achieve our aims, we applied modern molecular biology techniques such as the oligonucleotide microarray. Among the antioxidant defense genes, five (SOD1, PLK3, CLN8, XPA, HAO1), whose expression was significantly altered by the action of fluoride ions and the exposure to SMF were normalized their expression was identified. We showed that fluoride ions cause oxidative stress, whereas exposure to SMF with a moderate induction can suppress their effects by normalizing the expression of the genes that are altered by fluoride. Our research may explain the molecular mechanisms of the influence of fluoride and SMF that are generated by permanent magnets on cells.
Inne
System-identifier
PX-5b5af9bdd5dec1f320073b64
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