Biological effects in photodynamic treatment combined with electropermeabilization in wild and drug resistant breast cancer cells
PBN-AR
Instytucja
Instytut Immunologii i Terapii Doświadczalnej im. Ludwika Hirszfelda Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
BIOELECTROCHEMISTRY (35pkt w roku publikacji)
ISSN
1567-5394
EISSN
Wydawca
ELSEVIER SCIENCE SA
DOI
URL
Rok publikacji
2018
Numer zeszytu
Strony od-do
9-18
Numer tomu
123
Liczba arkuszy
Autorzy
(liczba autorów: 6)
Pozostali autorzy
+ 5
Słowa kluczowe
angielski
Breast cancer
Cyanine
Electro-photodynamic therapy
Electroporation
Multidrug resistance
Photodynamic therapy
Streszczenia
Język
angielski
Treść
Electrochemotherapy became one of the therapeutic protocols successfully used in oncology. However, biological effects occurring in cells, especially those which are drug resistant, have not been studied thoroughly. This study presents response of wild and drug resistant breast cancer cells to classical photodynamic therapy with Photofrin or experimental photodynamic therapy with cyanine IR-775, combined with electroporation. Photodynamic reaction or electroporation alone had no cytotoxic effect, but their combination significantly disturbed cellular functions. Applying electroporation allowed the drugs to increase its accumulation, especially for a poorly permeant cyanine in drug resistant cells. FACS analysis showed that even at relatively mild electric field, ca. 90% of cells were permeabilized. High intracellular concentration of drugs triggered the cellular defense system through increased expression of glutathione S-transferase and multidrug resistance proteins (MDR1 and MRP7), particularly in drug resistant cells. Finally, expressively decreased cell metabolism and proliferation, as well as formation of apoptotic bodies and fragmentation of cells were observed after the combined treatment. The results show that electroporation can be used for effective delivery of photosensitizers, even to drug resistant breast cancer cells, which was not tested before. This shows that electro-photodynamic treatment could be a promising approach to overcome a problem of drug resistance in cancer cells.
Cechy publikacji
artykuł oryginalny
Inne
System-identifier
PX-5bc4898ed5de664af89209de
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