Biopolymers conjugated with magnetite as support materials for trypsin immobilization and protein digestion
PBN-AR
Instytucja
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Colloids and Surfaces B: Biointerfaces (35pkt w roku publikacji)
ISSN
0927-7765
EISSN
Wydawca
Elsevier B.V.
DOI
Rok publikacji
2018
Numer zeszytu
Strony od-do
118-125
Numer tomu
169
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 6)
Pozostali autorzy
+ 5
Słowa kluczowe
en
Enzyme immobilization
Hybrid supports
Magnetic materials
Peptide digestion
Trypsin
Streszczenia
Język
en
Treść
In the presented study synthesized magnetic nanoparticles were used as an inorganic precursor for the preparation of novel magnetite-lignin and magnetite-chitin hybrid supports for enzyme immobilization. Effective synthesis of the hybrids was confirmed by Fourier transform infrared spectroscopy and powder X-ray diffraction analysis. The materials exhibited good thermal stability and surface areas of 4.3 and 5.6 m2/g respectively. The magnetite-lignin + trypsin and magnetite-chitin + trypsin systems were found to have good storage stability and reusability. After 20 days they retained over 75% and 90% respectively of their initial activity, and after 10 consecutive biocatalytic cycles retained over 60% and 80% respectively of their initial activity. The kinetic parameters of the free and immobilized enzyme were also comprehensively examined and compared. The results of peptide digestion tests confirmed the high proteolytic activity of the produced trypsin-based magnetic biocatalytic systems.
Inne
System-identifier
PX-5b1f8003d5de79ce9d020bc9
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