Development of a novel keratin dressing which accelerates full-thickness skin wound healing in diabetic mice: In vitro and in vivo studies.
PBN-AR
Instytucja
Instytut Biochemii i Biofizyki Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
JOURNAL OF BIOMATERIALS APPLICATIONS (30pkt w roku publikacji)
ISSN
0885-3282
EISSN
Wydawca
SAGE PUBLICATIONS LTD
DOI
URL
Rok publikacji
2018
Numer zeszytu
4
Strony od-do
527-540
Numer tomu
33
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
EN
Chronic wounds;
diabetic mouse model;
full-thickness wound;
insoluble keratin; keratin dressing;
wound healing
Streszczenia
Język
EN
Treść
Impaired wound healing is a major medical problem in diabetes. The objective of this study was to determine the possible application of an insoluble fraction of fur-derived keratin biomaterial as a wound dressing in a full thickness surgical skin wound model in mice ( n=20) with iatrogenically induced diabetes. The obtained keratin dressing was examined in vitro and in vivo. In vitro study showed the keratin dressing is tissue biocompatible and non-toxic for murine fibroblasts. Antimicrobial examination revealed the keratin dressing inhibited the growth of S. aureus and E. coli. In vivo studies showed the obtained dressing significantly ( p<0.05) accelerated healing during the first week after surgery compared to control wounds. Keratin dressings were incorporated naturally into granulation and regenerating tissue without any visible signs of inflammatory response, which was confirmed by clinical and histopathological analysis. It is one of the first studies to show application of insoluble keratin proteins and its properties as a wound dressing. The obtained keratin dressing accelerated wound healing in mice with iatrogenically induced diabetes. Therefore, it can be considered as a safe and efficient wound dressing. Although future studies are needed to explain the molecular mechanism behind fur-derived keratin effect during the multilayer wound healing process, our findings may open the way for a new class of insoluble fur keratin dressings in chronic difficult to heal wounds treatment.
Inne
System-identifier
PX-5bd1d48cd5deb089ac1b49ce
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