Analysis of AGP contribution to the dynamic assembly and mechanical properties of cell wall during pollen tube growth
PBN-AR
Instytucja
Instytut Agrofizyki im. Bohdana Dobrzańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
PLANT SCIENCE
ISSN
0168-9452
EISSN
Wydawca
ELSEVIER IRELAND LTD
DOI
URL
Rok publikacji
2019
Numer zeszytu
Strony od-do
9-18
Numer tomu
281
Identyfikator DOI
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Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
angielski
AFM
Arabinogalactan protein
Callose
Cellulose
Fragaria x ananassa
Immunocytochemistry
Pectin
Streszczenia
Język
angielski
Treść
Arabinogalactan proteins as cell wall structural proteins are involved in fundamental processes during plant development and growth. The aim of this study was to evaluate AGP function in the distribution of pectin, cellulose and callose along Fragaria x ananassa pollen tube and to associate the cell wall structure with local mechanical properties. We used Yariv reagent which interacts with AGPs and allows the observation of the assembly of cell walls without AGPs performing their function. Cytochemical, immunofluorescence labelling and atomic force microscope have been used to characterize the changes in cell wall structure and stiffness. It was shown that disordering of the structure of AGP present in cell walls affects the localization of cellulose, pectins and the secretion of callose. Changes in cell wall assembly are relevant to pollen tube mechanical properties. The stiffness gradient lengthwise through the axis of the pollen tube has demonstrated a significantly higher Young’s modulus of the shank region than the growth zone. It has been revealed that the apex of the pollen tube cultured in the presence of Yariv reagent is stiffer (1.68 MPa) than the corresponding region of the pollen tube grown under control conditions (0.13-0.27 MPa). AGP affects the structure of the cell wall by changing the distribution of other components and the modification of their localization, and hence it plays a significant role in the mechanical properties of the cell wall.
Cechy publikacji
original_article
Inne
System-identifier
PX-5c4043f9d5dea169047bbde4
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