AFM‐based nanomechanical characterization of bronchoscopic samples in asthma patients
PBN-AR
Instytucja
Instytut Fizyki Jądrowej im. Henryka Niewodniczańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF MOLECULAR RECOGNITION (25pkt w roku publikacji)
ISSN
0952-3499
EISSN
Wydawca
DOI
URL
Rok publikacji
2018
Numer zeszytu
12
Strony od-do
e2752
Numer tomu
31
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 9)
Pozostali autorzy
+ 7
Słowa kluczowe
en
Asthma
Atomic force microscopy
Bronchoscopic samples
Tissue elasticity
Streszczenia
Język
en
Treść
Asthma is not a single disease, but recently, it is considered as a syndrome characterized through various clinical presentations and different etiopathologies. Large degree of the disease heterogeneity manifests in distinct characteristics that translate into variability of properties at single cell and molecular levels. Here, we conducted measurements of mechanical properties of bronchial tissue samples collected from patients suffering from asthma. The results obtained from different applied protocols for sample preparation may indicate that deep freezing and storage in liquid nitrogen, followed by consecutive unfreezing of tissue samples, preserve tissue mechanical properties as indicated by a parameter referred here as a tissue relative stiffness index. Tissue relative stiffness index quantifies both the degree of heterogeneity and deformability of tissue samples regarding healthy one. These studies demonstrate that the freezing protocol, optimized towards asthma tissue, can facilitate atomic force microscopy use what, together with recent findings on standardization of elasticity measurements, enables the measurements of large group of samples with minimized influence of errors stemming from the applied methodology of tissue stiffness determination.
Cechy publikacji
peer-reviewed
original-article
Inne
System-identifier
30358/2018
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