Deletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII
PBN-AR
Instytucja
Wydział Biologii (Uniwersytet Warszawski)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
PLANT MOLECULAR BIOLOGY (40pkt w roku publikacji)
ISSN
0167-4412
EISSN
1573-5028
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2018
Numer zeszytu
Strony od-do
135-149
Numer tomu
96
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Chloramphenicol acetyltransferase; Cyanidioschyzon merolae; DTA toxin; PEG; PSII extrinsic protein deletion; PSII mutants; PsbQ’; Stable red algae nuclear transformation
Streszczenia
Język
en
Treść
We have successfully produced single-cell colonies of C. merolae mutants, lacking the PsbQ' subunit in its PSII complex by application of DTA-aided mutant selection. We have investigated the physiological changes in PSII function and structure and proposed a tentative explanation of the function of PsbQ' subunit in the PSII complex. We have improved the selectivity of the Cyanidioschyzon merolae nuclear transformation method by the introduction of diphtheria toxin genes into the transformation vector as an auxiliary selectable marker. The revised method allowed us to obtained single-cell colonies of C. merolae, lacking the gene of the PsbQ' extrinsic protein. The efficiency of gene replacement was extraordinarily high, allowing for a complete deletion of the gene of interest, without undesirable illegitimate integration events. We have confirmed the absence of PsbQ' protein at genetic and protein level. We have characterized the physiology of mutant cells and isolated PSII protein complex and concluded that PsbQ' is involved in nuclear regulation of PSII activity, by influencing several parameters of PSII function. Among these: oxygen evolving activity, partial dissociation of PsbV, regulation of dimerization, downsizing of phycobilisomes rods and regulation of zeaxanthin abundance. The adaptation of cellular physiology appeared to favorite upregulation of PSII and concurrent downregulation of PSI, resulting in an imbalance of energy distribution, decrease of photosynthesis and inhibition of cell proliferation.
Cechy publikacji
discipline:Biochemia – dziedzina nauk biologicznych
discipline:Biologia
discipline:Biotechnologia – dziedzina nauk biologicznych
discipline:Biochemistry – field of biological sciences
discipline:Biology
discipline:Biotechnology – field of biological sciences
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:847919
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