Draft genome sequence of an Escherichia coli ST410 isolate co-harbouring blaCTX-M-15, blaCMY-42, blaOXA-1, aac(3)-IIa and aac(6′)-Ib-cr genes with gyrA and parC mutations isolated from a paediatric patient in Poland
PBN-AR
Instytucja
Narodowy Instytut Zdrowia Publicznego - Państwowy Zakład Higieny
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Global Antimicrobial Resistance
ISSN
2213-7165
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
Rok publikacji
2019
Numer zeszytu
Strony od-do
120 - 122
Numer tomu
16
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
ST410
CTX-M-15
CMY-42
-cr
Whole-genome sequencing
Streszczenia
Język
en
Treść
Objectives Escherichia coli is one of the major causative agents of nosocomial infections. Here we report the first draft genome sequence of an E. coli strain (no. 158) isolated in Poland carrying blaCTX-M-15, blaCMY-42, blaOXA-1, aac(3)-IIa and aac(6′)-Ib-cr genes together with mutations in the gyrA and parC genes. Methods Total DNA was sequenced using an Illumina NextSeq 500 platform. The draft genome of E. coli strain 158 was assembled using SPAdes 3.9 assembler. Contigs were annotated using the Prokka v.1.12 algorithm. Species confirmation, multilocus sequence typing (MLST), serotyping, molecular virulence and resistance traits, and plasmid replicons were analysed using appropriate bioinformatics tools available at the Centre for Genomic Epidemiology website. Additional in silico analyses were also conducted. Result The genome size was estimated at 4883487bp, with 4601 predicted coding sequences. The presence of blaCTX-M-15, blaCMY-42, blaOXA-1, aac(3)-IIa and aac(6′)-Ib-cr genes was detected in addition to other antimicrobial resistance genes as well as mutations in the gyrA (Ser83Leu and Asp87Asn) and parC (Ser80Ile) genes. The investigated strain E. coli 158 belongs to ST410. Conclusion To our knowledge, this is the first draft genome of an E. coli strain co-harbouring blaCTX-M-15, blaCMY-42, blaOXA-1, aac(3)-IIa and aac(6′)-Ib-cr genes with mutations in gyrA and parC reported in Poland. The reported genome sequence contains valuable information on genetic features of antimicrobial resistance mechanisms of E. coli in Poland.
Cechy publikacji
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:909043
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