Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis
PBN-AR
Instytucja
Instytut Rybactwa Śródlądowego im. Stanisława Sakowicza
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Journal of Applied Genetics (20pkt w roku publikacji)
ISSN
1234-1983
EISSN
2190-3883
Wydawca
SPRINGER HEIDELBERG
DOI
URL
Rok publikacji
2018
Numer zeszytu
Strony od-do
91-97
Numer tomu
59
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 5)
Pozostali autorzy
+ 3
Słowa kluczowe
EN
Chromosome fragments
Gynogenesis
Heterologous sperm
Hybrids
High hydrostatic pressure
UV radiation
Streszczenia
Język
EN
Treść
Rainbow trout (Oncorhynchus mykissWalbaum) and sea trout (Salmo trutta Linnaeus, 1758) show large karyotypic differences and their hybrid offspring is not viable due to unstable karyotype and chromosome fragmentation. However, gametes from these two species were used to induce gynogenetic development. Rainbow trout eggs activated by UV-irradiated sea trout sperm were subjected to high hydrostatic pressure (HHP) shock to prevent release of the 2nd polar body (early shock) or to inhibit the first cleavage (late shock) in order to produce diploid meiotic gynogenotes and gynogenetic doubled haploids (DHs), respectively. Cytogenetic analysis proved fish that development was induced by the sea trout spermatozoa were rainbow trout. In turn, molecular examination confirmed homozygosity of the gynogenetic DHs. Presumed appearance of the recessive alleles resulted in lower survival of the gynogenetic DH larvae (~25%) when compared to survival of the heterozygous (meiotic) gynogenotes (c. 50%). Our results proved that genomic incompatibilities between studied trout species result in the hybrid unviability. However, artificial gynogenesis including activation of rainbow trout eggs with UV-irradiated sea trout spermatozoa was successfully induced. As both species are unable to cross, application of the UV-irradiated sea trout spermatozoa to activate rainbow trout development assures only maternal inheritance with no contamination by the residues of the paternal chromosomes.
Cechy publikacji
ORIGINAL PAPER
Inne
System-identifier
PX-5c66816fd5de256646f87356
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