Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods
PBN-AR
Instytucja
Instytut Chemii Fizycznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
SCIENTIFIC REPORTS OF THE NATURE PUBLISHING GROUP
ISSN
2045-2322
EISSN
Wydawca
DOI
URL
Rok publikacji
2019
Numer zeszytu
Strony od-do
8575
Numer tomu
9
Identyfikator DOI
Liczba arkuszy
Streszczenia
Język
en
Treść
Evolution of bacteria to selective chemical pressure (e.g. antibiotics) is well studied in contrast to the influence of physical stressors. Here we show that instantaneous physical stress in a homogeneous environment (without concentration gradient) induces fast adaptation of Escherichia coli. We exposed E. coli to a large number of collisions of around 105 per bacterium per second with sharp ZnO nanorods. The pressure exerted on the bacterial cell wall was up to 10 GPa and induced phenotype changes. The bacteria's shape became more spherical, the density of their periplasm increased by around 15% and the average thickness of the cell wall by 30%. Such E. coli cells appeared almost as Gram-positive bacteria in the standard Gram staining. Additionally, we observed a combination of changes occurring at the genomic level (mutations identified in form of single nucleotide polymorphisms) and down-regulation of expression of 61 genes encoding proteins involved in β-oxidation of fatty acids, glycolysis, the citric acid cycle, as well as uptake of amino acids and enzyme cofactors. Thus, we show that bacteria undergo phenotypic changes upon instantaneous, acute physical stress without any obviously available time for gradual adaptation.
Cechy publikacji
discipline:Biochemia – dziedzina nauk biologicznych
discipline:Biochemia – dziedzina nauk chemicznych
discipline:Biofizyka – dziedzina nauk biologicznych
discipline:Biofizyka – dziedzina nauk fizycznych
discipline:Biologia
discipline:Biotechnologia – dziedzina nauk biologicznych
discipline:Biotechnologia – dziedzina nauk chemicznych
discipline:Chemia
discipline:Mikrobiologia
discipline:Biochemistry – field of biological sciences
discipline:Biochemistry – field of chemical sciences
discipline:Biophysics – field of biological sciences
discipline:Biophysics – field of physical sciences
discipline:Biology
discipline:Biotechnology – field of biological sciences
discipline:Biotechnology – field of chemical sciences
discipline:Chemistry
discipline:Microbiology
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:916305
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