Biomolecular influenza virus detection based on the electrochemical impedance spectroscopy using the nanocrystalline boron-doped diamond electrodes with covalently bound antibodies
PBN-AR
Instytucja
Wydział Chemii (Uniwersytet Gdański)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Sensors and Actuators B - Chemical
ISSN
0925-4005
EISSN
Wydawca
Elsevier S.A.
DOI
Rok publikacji
2019
Numer zeszytu
Strony od-do
263-271
Numer tomu
280
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 8
Autorzy przekładu
(liczba autorów przekładu: 0)
Słowa kluczowe
en
Impedance spectroscopy
influenza virus
antibodies
protein
BDD electrode
nanocrystalline boron-doped diamond electrodes
diamond electrode modification
Streszczenia
Język
en
Treść
New rapid pathogen detection methods with improved cost-effectiveness and efficiency are currently in the focus of the scientists from all over the world. Based on the experiences from the rapid spread of the influenza virus pandemic in 2009 it is clear that the development of the system for early diagnosis of this infection is essential. The crucial stage of the treatment is the detection of the viral infection during its initial development phase when approximately only several dozens to hundreds of virus particles are present in the pharynx of the infected individual. The present study describes the new universal antibody-modified nanocrystalline boron-doped diamond biosensor for the direct detection of the viral particles at ultralow concentrations. Polyclonal anti-M1 antibodies (aM1) against the M1 protein, the universal biomarker of influenza virus, are attached to the surface of the diamond electrode. Capturing the M1 protein results in electrochemical impedance spectra changes. Achieved limit of detection (LOD) for the approach using M1 biomarker in saliva buffer is 5×10−14 g/mL, i.e., several virus particles per sample. Additionally, this assay may be soon developed into the first commercial test utilizing diamond electrodes for the detection of the influenza infection.
Inne
System-identifier
UOGceb49ec9233c4144acc6d6d34d7c626c
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych