Nanocrystalline hydroxyapatite loaded with resveratrol in colloidal suspension improves viability, metabolic activity and mitochondrial potential in human adipose-derived mesenchymal stromal stem cells (hASCs)
PBN-AR
Instytucja
Wydział Biologii i Hodowli Zwierząt (Uniwersytet Przyrodniczy we Wrocławiu)
Informacje podstawowe
Główny język publikacji
Czasopismo
Polymers
ISSN
2073-4360
EISSN
Wydawca
DOI
URL
Rok publikacji
2019
Numer zeszytu
1
Strony od-do
1-23
Numer tomu
11
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Autorzy
Pozostali autorzy
+ 4
Słowa kluczowe
angielski
hydrogels
3,6-anhydro-α-L-galacto-β-D-galactan
resveratrol; nanocomposites
Open access
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Creative Commons — Uznanie autorstwa
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Streszczenia
Język
angielski
Treść
In response to the demand for new multifunctional materials characterized by high biocompatibility, hydrogel (HG) nanocomposites as a platform for bioactive compound delivery have been developed and fabricated. A specific crosslinking/copolymerization chemistry was used to construct hydrogels with a controlled network organization. The hydrogels were prepared using 3,6-anhydro-α-L-galacto-β-D-galactan (galactose hydrogel) together with resveratrol (trans-3,5,40 -trihydroxystilbene) and calcium hydroxyapatite nanoparticles. The resveratrol was introduced in three different concentrations of 0.1, 0.5, and 1 mM. Nanosized calcium hydroxyapatite was synthesized by a microwave-assisted hydrothermal technique, annealed at 500 ◦C for 3 h, and introduced at a concentration 10% (m/v). The morphology and structural properties of Ca10(PO4)6(OH)2 and its composite were determined by using XRPD (X-ray powder diffraction) techniques, as well as the absorption and IR (infrared) spectroscopy. The average nanoparticle size was 35 nm. The water affinity, morphology, organic compound release profile, and cytocompatibility of the obtained materials were studied in detail. The designed hydrogels were shown to be materials of biological relevance and of great pharmacological potential as carriers for bioactive compound delivery. Their cytocompatibility was tested using a model of human multipotent stromal cells isolated from adipose tissue (hASCs). The biomaterials increased the proliferative activity and viability of hASCs, as well as reduced markers of oxidative stress. In light of the obtained results, it has been thought that the designed materials meet the requirements of the tissue engineering triad, and may find application in regenerative medicine, especially for personalized therapies.
Inne
System-identifier
PX-5c864445d5de6f9b442730d3
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