An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue
PBN-AR
Instytucja
Wydział Fizyki (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Nukleonika (15pkt w roku publikacji)
ISSN
0029-5922
EISSN
Wydawca
DOI
URL
Rok publikacji
2017
Numer zeszytu
2
Strony od-do
159-163
Numer tomu
62
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 4
Słowa kluczowe
en
Mössbauer spectroscopy
background spectrum
iron concentration
brain tissue
calibration curve
Streszczenia
Język
en
Treść
Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets - a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 μg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result.
Inne
System-identifier
WUT9e9895cd05b5407f945700eec176163f
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