Biophysical and Biochemical Characteristics as Complementary Indicators of Melanoma Progression
PBN-AR
Instytucja
Instytut Fizyki Jądrowej im. Henryka Niewodniczańskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
ANALYTICAL CHEMISTRY
ISSN
0003-2700
EISSN
Wydawca
AMER CHEMICAL SOC
DOI
URL
Rok publikacji
2019
Numer zeszytu
15
Strony od-do
9885-9892
Numer tomu
91
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 5
Autorzy przekładu
(liczba autorów przekładu: 0)
Słowa kluczowe
en
Biochemical characteristics
Biochemical properties
Biomechanical properties
Cancer progression
Complementary techniques
Nanoscale levels
Tissue properties
Tumor progressions
Streszczenia
Język
en
Treść
The multistep character of cancer progression makes it difficult to define a unique biomarker of the disease. Interdisciplinary approaches, combining various complementary techniques, especially those operating at a nanoscale level, potentially accelerate characterization of cancer cells or tissue properties. Here, we study a relation between the surface and biomechanical properties of melanoma cells, measured by mass spectrometry (ToF-SIMS) and atomic force microscopy (AFM). In total, seven cell lines have been studied. Six of them were melanoma cells derived from various stages of tumor progression: (1) WM115 cells derived from a 55 year old female skin melanoma at a vertical growth phase (VGP) in the primary melanoma site, (2) WM793 cells established from the vertical growth phase (VGP) of a primary skin melanoma lesion, (3) WM266-4 cells established from a cutaneous skin metastasis detected in the same patient as WM115 cells, (4) WM239 cells derived from a cutaneous skin metastasis, (5) 1205Lu cells originated from a lung metastasis diagnosed in the same patient as WM793 cells, and (6) A375P-cells were derived from a solid malignant tumor located in the lung. As a reference cell line, human epidermal melanocytes from adult skin (primary cell line HEMa-LP) were used. Results reveal low, medium, and large deformability of melanoma cells originating from vertical growth phase (VGP), and skin and lung metastasis, respectively. These changes were accompanied by distinct outcome from principal component analysis (PCA). In relation to VGP melanoma cells, cells from skin and lung metastasis reveal similar or significantly different surface properties. The largest deformability difference observed for cells from VGP and lung metastasis was accompanied by the largest separation of unspecific changes in their surface properties. In this way, we show the evidence that biomechanical and surface biochemical properties of cells change in parallel, indicating a potential of being used as nanobiophysical fingerprints of melanoma progression.
Cechy publikacji
peer-reviewed
original-article
Inne
System-identifier
31433/2019
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