AFM and QCM-D as tools for the distinction of melanoma cells with a different metastatic potential
PBN-AR
Instytucja
Wydział Chemiczny (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Biosensors & Bioelectronics (40pkt w roku publikacji)
ISSN
0956-5663
EISSN
Wydawca
Elsevier BV
DOI
URL
Rok publikacji
2017
Numer zeszytu
Strony od-do
274-281
Numer tomu
93
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
Atomic force microscopy
Quartz crystal microbalance
Melanoma cells
Lectin-carbohydrate interaction
Streszczenia
Język
en
Treść
Malignant melanoma is one of the most dangerous skin cancer originating from melanocytes. Thus, an early and proper melanoma diagnosis influences significantly the therapy efficiency. The melanoma recognition is still difficult, and generally, relies on subjective assessments. In particular, there is a lack of quantitative methods used in melanoma diagnosis and in the monitoring of tumour progression. One such method can be the atomic force microscopy (AFM) working in the force spectroscopy mode combined with quartz crystal microbalance (QCM), both applied to quantify the molecular interactions. In our study we have compared the recognition of mannose type glycans in melanocytes (HEMa-LP) and melanoma cells originating from the radial growth phase (WM35) and from lung metastasis (A375-P). The glycosylation level on their surfaces was probed using lectin concanavalin A (Con A) from Canavalia ensiformis. The interactions of Con A with surface glycans were quantified with both AFM and QCM techniques that revealed the presence of various glycan structural groups in a cell-dependent manner. The Con A – mannose (or glucose) type glycans present on WM35 cell surface are rather short and less ramified while in A375-P cells, Con A binds to long, branched mannose and glucose types of oligosaccharides.
Inne
System-identifier
WUTe9bdf7e0f1b544d38894eccb802cdc85
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych