Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench
PBN-AR
Instytucja
Instytut Włókien Naturalnych i Roślin Zielarskich
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN
1054-5476
EISSN
1475-2689
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2019
Numer zeszytu
Strony od-do
190-198
Numer tomu
55
Identyfikator DOI
Liczba arkuszy
1,1
Słowa kluczowe
angielski
Sorghum bicolor
Callus induction
Shoot regeneration
Honey
Open access
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Wersja opublikowana
Licencja otwartego dostępu
Creative Commons — Uznanie autorstwa
Czas opublikowania w otwartym dostępie
Przed publikacją
Data udostępnienia w sposób otwarty
2019-02-26
Streszczenia
Język
angielski
Treść
Sorghum bicolor is a recalcitrant species for tissue culture regeneration and genetic transformation. Browning of explants is one of the factors limiting organ and tissue cultures. To overcome this, callus tissue was initiated from the shoot tips of in vitro germinating seeds (S. bicolor cv. Róna 1), and then cultured on modified MS media (Murashige and Skoog in Physiol Plant 15:473–497, 1962). In the first experiment, we tested callus induction on several media supplemented with casein hydrolysate, polyvinylpyrrolidone, honey, and sucrose. The best callus induction was recorded for the medium with honey and sucrose (80.0%) and for control medium (79.8%). Shoot regeneration was tested on the MS medium with 6-benzylaminopurine (BAP) supplemented with honey and sucrose at a 1:1 ratio (by weight) or with sucrose only. The highest percentage of calluses regenerating shoots was noted for those induced on the medium with sucrose and honey—approx. four times higher when compared to the control. Rooted plantlets were acclimatized with a 92% survival rate. In the second experiment, we analyzed culture responses to various ways of honey application to the induction media: honey (autoclaved or filtered) in presence or absence of sucrose. Supplementation of the medium with fructose, glucose, and maltose at a proportion typical for honey was also investigated. The explant and callus survival rates were similar to those of the honey–sucrose combination in the first experiment. Only presence of both sucrose and honey in the induction medium improved the total regeneration rateto 37.9% over the control (18.8%). Sucrose and honey appear to act synergistically for shoot regeneration in callus cultures of sorghum.
Inne
System-identifier
PX-5d6fa8dfd5de71884df3267a
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