Blebbistatin reveals beneficial effects on the cystometric parameters in an animal model of detrusor overactivity
PBN-AR
Instytucja
Wydział Biologii i Biotechnologii (Uniwersytet Marii Curie-Skłodowskiej w Lublinie)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
ISSN
0028-1298
EISSN
1432-1912
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2019
Numer zeszytu
7
Strony od-do
843-850
Numer tomu
392
Identyfikator DOI
Liczba arkuszy
Streszczenia
Język
angielski
Treść
The aims of the study were to determine the effectiveness of blebbistatin (BLEB) on detrusor overactivity (DO) in an animal model induced by retinyl acetate (RA) and, because of potential urothelial permeability, to evaluate the degenerative impact of BLEB on the urothelium. Three days after RA instillation into the urinary bladder, BLEB was administered into the bladder and immediately after cystometric assessment was performed. Furthermore, Evans Blue extravasation into bladder tissue and urothelium thickness were measured. Sixty female Wistar rats were used and randomly assigned to one of four groups (n = 15 in each group): (1) control, (2) RA, (3) BLEB, and (4) RA + BLEB. RA administration induced changes in cystometric parameters reflecting DO, as previously reported. Treatment with BLEB did not significantly alter cystometric parameters in rats which did not receive RA. Administration of BLEB to rats pretreated with RA reversed changes in cystometric parameters induced by RA in basal pressure, threshold pressure, detrusor overactivity index, amplitude of nonvoiding contractions, frequency of nonvoiding contractions, voided volume, volume threshold, intercontraction interval, bladder compliance, and volume threshold to elicit nonvoiding contractions. There were no significant differences in Evans Blue extravasation into bladder tissue or urothelium thickness between the groups. The current research provides new data on the possible utility of blebbistatin in the pharmacotherapy of DO, which is an important feature of overactive bladder (OAB). Further studies in human patients with DO/OAB are warranted to confirm these preclinical results.
Inne
System-identifier
PX-5d863688d5de4731819824fa
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