Xanthohumol exhibits anti-myeloma activity in vitro through inhibition of cell proliferation, induction of apoptosis via the ERK and JNK-dependent mechanism, and suppression of sIL-6R and VEGF production
PBN-AR
Instytucja
Wydział Biologii i Biotechnologii (Uniwersytet Marii Curie-Skłodowskiej w Lublinie)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
ISSN
0304-4165
EISSN
1872-8006
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2019
Numer zeszytu
11
Strony od-do
129408 (1-14)
Numer tomu
1863
Identyfikator DOI
Liczba arkuszy
Streszczenia
Język
angielski
Treść
Background Xanthohumol (XN, a hop-derived prenylflavonoid) was found to exert anticancer effects on various cancer types. However, the mechanisms by which XN affects the survival of multiple myeloma cells (MM) are little known. Therefore, our study was undertaken to address this issue. Methods Anti-proliferative activity of XN towards two phenotypically distinct MM cell lines U266 and RPMI8226 was evaluated with the MTT and BrdU assays. Cytotoxicity was determined with the LDH method, whereas apoptosis was assessed by flow cytometry and fluorescence staining. The expression of cell cycle- and apoptosis-related proteins and the activation status of signaling pathways were estimated by immunoblotting and ELISA assays. Results XN reduced the viability of RPMI8226 cells more potently than in U266 cells. It blocked cell cycle progression through downregulation of cyclin D1 and increased p21 expression. The marked apoptosis induction in the XN-treated RPMI8226 cells was related to initiation of mitochondrial and extrinsic pathways, as indicated by the altered p53, Bax, and Bcl-2 protein expression, cleavage of procaspase 8 and 9, and elevated caspase-3 activity. The apoptotic process was probably mediated via ROS overproduction and MAPK (ERK and JNK) activation as N-acetylcysteine, or specific inhibitors of these kinases prevented the XN-induced caspase-3 activity and, hence, apoptosis. Moreover, XN decreased sIL-6R and VEGF production in the studied cells. Conclusions ERK and JNK signaling pathways are involved in XN-induced cytotoxicity against MM cells. General Significance: The advanced understanding of the molecular mechanisms of XN action can be useful in developing therapeutic strategies to treat multiple myeloma.
Inne
System-identifier
PX-5d91ae95d5defb833b7fa7e5
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